MUTZ-3, a human cell line model for the cytokine-induced differentiation of dendritic cells from CD34+ precursors.

Brief report MUTZ-3, a human cell line model for the cytokine-induced differentiation of dendritic cells from CD34 precursors

Many human myeloid leukemia–derived cell lines possess the ability to acquire a dendritic cell (DC) phenotype. However, cytokine responsiveness is generally poor, requiring direct manipulation of intracellular signaling mechanisms for differentiation. In contrast, the CD34 human acute myeloid leukemia cell line MUTZ-3 responds to granulocyte macrophage– colony-stimulating factor (GM-CSF), inter...

A Human Cell Line Model for Interferon-α Driven Dendritic Cell Differentiation

The CD34+ MUTZ-3 acute myeloid leukemia cell line has been used as a dendritic cell (DC) differentiation model. This cell line can be cultured into Langerhans cell (LC) or interstitial DC-like cells using the same cytokine cocktails used for the differentiation of their primary counterparts. Currently, there is an increasing interest in the study and clinical application of DC generated in the ...

Human dendritic cell line models for DC differentiation and clinical DC vaccination studies.

Dendritic cells (DC) are increasingly applied in the immunotherapy of cancer. As the development of a standardized DC vaccine product is often hampered by the limited availability of DC precursors and inter- and intra-donor variability, and the preparation of individual vaccines is labor-intensive, it would be preferable to use DC from a readily available and unlimited source, such as cell line...

Enhanced dendritic cell development through long-term proteasome inhibition

Bortezomib (BTZ) is a reversible proteasome inhibitor. It affects NF-κB activity and has shown clinical efficacy in multiple myeloma. However, the same mechanisms through which it curtails malignant growth may compromise antitumor immunity. As dendritic cells (DC) play a vital role in the elicitation and maintenance of antitumor immunity, we studied the long-term effects of BTZ on human DC deve...

Transcriptional profiling of interstitial dendritic cells and Langerhans cells derived from the human CD34+ dendritic precursor cell line MUTZ-3